Prof. Dr. Silvia Domcke

Area of Research

Description of Research Interest

Biology is amazingly complex, and yet only a minuscule fraction of possible cell states are found in nature. Our lab is investigating the full potential of cellular diversity and how it can be regulated.
At the heart of cell fate determination are transcription factors (TFs), the master regulators of gene expression. We are seeking to predict de novo TF activity in any cell, to perform intentional cell state transitions. This includes exploring uncharted regions in the landscape of cell states, e.g. transitions from diseased to healthy. Our research combines advanced mammalian cell culture models, genetic and epigenetic editing, single cell genomics, large-scale CRISPR screens, and various computational approaches to achieve this goal.

Special Expertise

- single cell genomics
- large-scale single cell CRISPR screens
- epigenetics
- gene regulation and transcription factors
- genome engineering
- cell state transitions
- experimental and computational genomics
- stem cells, embryoid bodies, differentiation systems

Shareable Platforms, Services, Equipment & Infrastructure

- single cell genomics
- large-scale pooled single cell CRISPR screens